Automated analysis of morphological and synaptic characteristics in neurons
Johannes Hjorth (Vrije Universiteit Amsterdam), Sabine Schmitz (Vrije Universiteit Amsterdam)
Here we present SynD, a MATLAB based program, developed to automatically analyze dendrite and synapse characteristics in immuno-fluorentscence images. The program uses a combination of steerable filters and deconvolution to detect dendrites and synapses. It reports dendrite morphology, synapse size, number and distribution, as well as protein expression at the soma, at synapses and as a function of distance from the soma. The dendritic tree is characterized using Sholl analysis.
The automatic measures have been compared to manually traced neurons and synapses showing good accuracy. Results are exported as csv files readable by excel and to user-friendly summary figures.
Automated analysis of neuronal morphology, synapse number and synaptic recruitment.
Schmitz SK, Hjorth JJJ, et al 2011, J Neurosci Methods 195(2):185-93 doi:http://dx.doi.org/10.1016/j.jneumeth.2010.12.011