Independent components of reconstructed current sources reflect activity of individual cell populations
Helena Głąbska (Department of Neurophysiology, Nencki Institute), Jan Potworowski (Department of Neurophysiology, Nencki Institute), Szymon Łęski (Department of Neurophysiology, Nencki Institute), Daniel Wójcik (Department of Neurophysiology, Nencki Institute)
We used this model to study the information contained in independent components obtained from the reconstructed Current Source Density (CSD) , which smooths transmembrane currents, decomposed with Independent Component Analysis (ICA) . We found out that three components obtained reliably matched well the activity of two dominating cell populations: superior pyramidal cells in layer 2/3 (rhythmic spiking) and tufted pyramids from layer 5 (intrinsically bursting). Interestingly, the pyramidal population from layer 2/3 could not be well described as a product of spatial profile and temporal activation, but was matched well by a sum of two such products which we recovered in two of the ICA components in our analysis, which seem to reflect different inputs on dendritic trees within the population.
Research supported by grants 5428/B/P01/2010/39, POIG.02.03.00-00-003/09, POIG.02.03.00-00-018/08.
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